Gene tools morpholinos

A sensitive assay system to test antisense oligonucleotides for splice suppression therapy in the mouse liver. Blood Cell Molec Dis. Regulation of the extrinsic apoptotic pathway by microRNA in alcoholic liver injury.

J Hepatol. Transcription factors involved in retinogenesis are co-opted by the circadian clock following photoreceptor differentiation. De novo mutation in ATP6V1B2 impairs lysosome acidification and causes dominant deafness-onychodystrophy syndrome. Cell Res. Neurobiol Dis. Knockdown of mitogen-activated protein kinase MAPK signalling in the midgut of Anopheles stephensi mosquitoes using antisense morpholinos.

Insect Mol Biol. Lessons learned from vivo-morpholinos: How to avoid vivo-morpholino toxicity. Specific NuRD components are required for fin regeneration in zebrafish. BMC Biology. Inhibition of enterovirus 71 infection by antisense octaguanidinium dendrimer-conjugated morpholino oligomers.

Antiviral Res. Inhibition of the liver expression of arylalkylamine N-acetyltransferase increases the expression of angiogenic factors in cholangiocytes.

Hepatobiliary Surg Nutr. Arterioscler Thromb Vasc Biol. Clin Therap. Glutamate transporter GLT-1 mediates N-acetylcysteine inhibition of cocaine reinstatement. Addic Biol. J Appl Physiol. Chronic administration of the methylxanthine propentofylline impairs reinstatement to cocaine by a GLTdependent mechanism.

Therapeutic antagonists of microRNAs deplete leukemia-initiating cell activity. Epub Dec Society for Integrative and Comparative Biology The receptor interacting protein kinase 3 is a critical early mediator of acetaminophen-induced hepatocyte necrosis in mice. Khandekar G, Jagadeeswaran P. Role of hepsin in factor VII activation in zebrafish.

Blood Cells Mol Dis. Caveolin-1 upregulation in diabetic fibroblasts and wounded tissues: implication for understanding the underlying mechanisms of non-healing diabetic ulcers. Am J Physiol Endocrinol Metab. Reexpression of pyruvate kinase M2 in type 1 myofibers correlates with altered glucose metabolism in myotonic dystrophy. Identification of a colonial chordate histocompatibility gene.

PLoS Genet. Transcriptional regulation by the Wilms tumor protein, Wt1, suggests a role of the metalloproteinase Adamts16 in murine genitourinary development. Am J Hypertens. Dual suppression of hemangiogenesis and lymphangiogenesis by splice-shifting morpholinos targeting vascular endothelial growth factor receptor 2 KDR.

Regenerative neurogenesis from neural progenitor cells requires injury-induced expression of Gata3. Acute inflammation initiates the regenerative response in the adult zebrafish brain. It is noteworthy in this context that MO inhibition has been observed to last for more than 6 weeks. The novel growth factor, progranulin, stimulates mouse cholangiocyte proliferation via Sirtuin1-mediated inactivation of FOXO1.

Modulation of the biliary expression of arylalkylamine N-acetyltransferase alters the autocrine proliferative responses of cholangiocytes.

Cold Spring Harb Protoc. Upregulation of junctional adhesion molecule-A is a putative prognostic marker of hypertension. Cardiovasc Res. Protocadherin function in zebrafish retinal development. Dev Neurobiol. Fetuin-A acts as an endogenous ligand of TLR4 to promote lipid-induced insulin resistance. Nucleic Acid Therapeutics. Molecular target validation, antimicrobial delivery, and potential treatment of Toxoplasma gondii infections.

Bodywide skipping of exons 45—55 in dystrophic mdx52 mice by systemic antisense delivery. Vivo-morpholino knockdown of aquaporin 1 AQP1 protein expression in American eel Anguilla rostrata gastrointestinal tract.

Mitogen activated protein kinase regulates serum glucocorticoid kinase 1 during seawater acclimation in Atlantic killifish, Fundulus heteroclitus. Epub Apr 2.

Deep intron elements mediate nested splicing events at consecutive AG-dinucleotides to regulate alternative 3' splice site choice in vertebrate 4. Mol Cell Biol.

Functional characterization and targeted correction of ATM mutations identified in Japanese patients with ataxia-telangiectasia. Hum Mutat. Epub Nov 9. Pathogenic exon-trapping by SVA retrotransposon and rescue in Fukuyama muscular dystrophy. Use of vivo-morpholinos for control of protein expression in the adult rat brain. J Neurosci Meth. Intracranial injections in rat brains of oligos targeting three different RNA targets, some though cannula and some by direct injection.

NOTE: Kate Reissner informs us that the doses reported in this paper in nmol should have been reported in pmol; that is, the reported doses are x too high. The development of the adult intestinal stem cells: Insights from studies on thyroid hormone-dependent amphibian metamorphosis. Cell Biosci. Antitumorigenic potential of STAT3 alternative splicing modulation.

Oligos were administered 2x week for either 1 or 3 weeks. Suppression of the HPA axis during extrahepatic biliary obstruction induces cholangiocyte proliferation in the rat. Epub Oct 6. Mol Cell. A search for factors specifying tonotopy implicates DNER in hair-cell development in the chick's cochlea. Epub Apr 8. Vivo-Morpholinos locally injected in embryonic chick ears at E6.

Controlling gene loss of function in newts with emphasis on lens regeneration. Nature Protocols. An octaguanidine-morpholino oligo conjugate improves muscle function of mdx mice. Efficient in vivo manipulation of alternative pre-mRNA splicing events using antisense morpholinos in mice. Zebrafish von Willebrand factor. Transcription factor Sox11b is involved in spinal cord regeneration in adult zebrafish. Matsuda H, Shi YB. Stem Cells. Protein kinase D2 is a crucial regulator of tumour cell-endothelial cell communication in gastrointestinal tumours.

Vera T, Stec DE. Epub Jul Vivo-Morpholinos in mice. Kreidberg JA. WT1 and kidney progenitor cells. Knockdown of WT1 in murine kidney explants; review offering perspective on experiments in Hartwig et al. Abstract Morpholinos do not trigger cellular responses by binding to Toll-like receptors, eliminating a confounding artifact associated with knockdowns using siRNA.

Morpholino oligos are usually soluble at several millimolar aqueous concentration. An siRNA project often starts by screening sequences to find a reasonably effective one.

This was also a required step with phosphorothioate antisense oligos. Because of their well-understood mechanism of action and their ability to invade secondary structure, it is common to see effective knockdown from the first Morpholino oligo designed. Morpholino gene knockdowns have persisted as long as seven days after delivery into embryos, with similar long-term efficacy in cultured cells.

You should BLAST the sequences and then when you determine which sequences you want to have made enter those oligo sequences when you place your order. Skip to main content. Note: If using a credit card, it will be billed when you submit your order. Features of the Online Store Retains of shopping cart between browsing sessions.

Saves shipping and billing addresses for use in future orders. Epub: Not Epub. Abstract: Wnt11 family proteins are ligands that activate a type of Dishevelled-mediated, non-canonical Wnt signaling pathway. Loss of Delivery Method: microinjection. Organism or Cell Type: Xenopus laevis.

Wnt11 family dependent morphogenesis during frog gastrulation is marked by the cleavage furrow protein anillin. Citation: Cell Cycle. Epub: Yes. Organism or Cell Type: zebrafish. Esco2 and cohesin regulate CRL4 ubiquitin ligase ddb1 expression and thalidomide teratogenicity.

Cell Cycle.